Enumeration and characterization of Aeromonas spp. isolated from milk and some dairy products in Sharkia governorate Egypt

A survey was conducted to determine the prevalence of Aeromonas spp. in 75 random samples [25 each of raw cow's milk, local plain yoghurt and Domiati cheese] collected from different dairy shops, supermarket and street peddlers in Diarb Negm and Zagazig cities Sharkia Governorate, Egypt. Investigations involved proteolytic and lipolytic activities of isolated Aeromonas spp. and the effect of heat- treatment, acidity, pH and Sodium chloride concentration on prevalence of Aeromonas bacteria. Prevalence of Aeromonas spp. was proved in 32, 44 and 20.0% of examined raw cow's milk, local plain yoghurt and Domiati cheese samples with mean count of 9.8 x10[3], 1.4 x10[5] and 6.9 x10[3]/ml, respectively. Identification of confirmed raw cow's milk isolates revealed that A. trota, A. hydrophila, A. janda and A. caviae were the predominant strains with percentages of 40, 25, 25 and 10.0% respectively. While local plain yoghurt isolates could be identified as A. caviae, A. sobria, A. hydrophila, A. trota and A. schubertii with percentages of 36.4, 22.7, 18.2, 13.6 and 9.1% respectively. Meanwhile identification of 10 confirmed Domiati cheese cultures revealed that the predominant strains were A. hydrophila, A. caviae and A. trota with percentages of 30, 50 and 20% respectively. All laboratory pasteurized milk samples revealed no count and there is marked decrease in the count of Aeromonas spp. as the acidity% of the examined raw cow's milk samples increase. While the count decrease when the pH value of the examined local plain yoghurt samples decrease and the NaCl% of the examined Domiati cheese samples increase. Characterization of isolated Aeromonas strains pointed that 50% of A. hydrophila, 60% of A. caviae, 40% of A. sobria, 53.8% of A. Trota, 100% of A. janda and 50% of A. schubertii were psychrotrophic. A. hydrophila exhibited proteolytic and lipolytic activities at the percentage of 41.7 and 16.7% respectively but in case of A. caviae strains the percentages were 46.7% and 20% respectively and with A. trota were 30.8 and 15.4% respectively. 60% of A. sobria and 100% of A. janda and A. schubertii strains showed proteolytic activity only. The public health importance and economic significance of existing microorganisms as well as the suggestive measures for improving the quality of raw milk and dairy products were discussed

La miliaire tuberculeuse : a propos de 29 cas

La miliaire tuberculeuse (MT) est une forme grave de tuberculose; secondaire a la dissemination hematogene de Mycobacterium tuberculosis. Le but de ce travail est d'evaluer les aspects epidemiologiques; cliniques et evolutifs des MT. Il s'agit d'une etude retrospective portant sur 29 malades atteints de MT et ages en moyenne de 38 ans. Les principaux facteurs favorisants ont ete le diabete (24) et l'ethylisme (17). Les principaux signes respiratoires ont ete la toux; les expectorations et la dyspnee ; et extra-thoraciques; les cephalees; les signes urinaires et les douleurs abdominales. Sur la radiographie thoracique; l'image de miliaire a ete notee chez 72des malades. D'autres aspects associes ont ete notes a type de cavernes (24); d'adenopathies mediastinales (17;3) et d'opacites sequellaires (10;3). Les recherches de bacilles de Koch (BK) dans les crachats se sont revelees positives dans 55des cas. Le diagnostic positif a ete porte sur des donnees bacteriologiques dans 17 cas; histologiques dans 4 cas; des arguments de presomption dans 9 cas ; un patient ayant une confirmation bacteriologique et histologique. Le traitement a comporte quatre antituberculeux; sauf dans trois cas ou elle a ete triple. La corticotherapie a ete prescrite dans 6 cas. L'evolution a ete bonne dans 28 cas avec amelioration clinique et negativation des recherches de BK dans les crachats. Un deces est survenu suite a une hepatite fulminante. La MT necessite une demarche diagnostique reflective et une prise en charge rapide afin d'ameliorer son pronostic

Hydatic pulmonary embolism: about one case

Hydatic pulmonary embolism is rare which can be complicated by chronic cor pulmonale and respiratory chronic failure. A 37-year-old woman, who had been operated twice for hydatic cystic, was hospitalized for cough, exercise-induced dyspnea and vomic, which had developed over the previous month. The standard chest Xray was normal. Imaging findings [angioscanner and cardiac echography] showed a hydatic pulmonary embolism which started from hydatic cysts on the right heart. Hydatic pulmonary embolism is due to the spread hydatic elements in the pulmonary circulation. It results from the rupture of hydatic cyst on the right heart. Clinical manifestations are dominated by cough, hemoptysis, vomic and exercise-induced dyspnea. Positive diagnosis has benefited from imaging especially angioscanner and cardiac echographic findings. Prognosis is particularly poor. The treatment is mainly surgical aiming at curing the primal cyst

[Actinomyces peripheral pulmonary nodule]

Actinomycosis is a chronic suppurative infection usually caused by Actinomyces israelii. Pulmonary involvement is a rare condition. The diagnosis is often delayed because of various appearances and the difficulty in microbiologic identification. We report a case of pulmonary actinomycosis in a 58 year old man who presented training respiratory symptoms. Clinical and radiological picture was mimic lung cancer. The definite was made after thoracic surgery. The patient's clinical condition improved with antibiotic therapy based on penicillin G then amoxicillin for 6 months. Through this new case, we discuss the possibilities of improvement of diagnosis and therapeutic approach of pulmonary actinomycosis by reducing of invasives procedures

new brevifolin carboxylic acid derivative from lagerstroemia indica L.: isolation, characterization and antibacterial activity

A new polyphenolic, 1-O-brevifolin carboxylyl-beta-[4]C1 glucopyranose 5, together with four known phenolics, 2,6-di-O-galloyl- beta -[4]C1-glucopyranose 1, vescalagin 2, castalagin 3 and 3, 4, 8, 9, 10-pentahydroxydibenzo [b, d] pyran-6-one 4 have been isolated from the aqueous alcohol extract of the leaves of Lagerstroemia indica L. All structures were established on the basis of analysis of [1]H, [13]C NMR, HMQC, HMBC and ESI-MS spectral data. The extract as well as the isolates 1-5 showed considerable antibacterial activity against drug-resistant bacterial strains

Open reduction and internal fixation of displaced intra-articular calcaneal fractures

Twenty- six patients with 29 closed displaced intra-articular calcaneal fractures were treated by open reduction and internal fixation at Tanta University Hospital in the period from July 2001 till October 2003. Sixteen patients were males and ten were females. The age ranged from 20 to 45 years with an average of 33 year. The fractures were classified according to Sanders' classification into type II in 7 fractures, type III in 13 fractures, and type IV in 9 fractures. Internal fixation was performed using interfragmentary screws and specific plates for calcaneal fractures. The follow up period ranged from 7 to 20 months with an average of 10 months. The functional outcome was satisfactory in 20 fractures and unsatisfactory in 9 fractures. The complications met with were sural nerve injury in two cases, delayed wound healing in one case, broadening of the heel in one case, mal-reduction with residual varus deformity in two cases, peroneal tendinitis in four eases, deep infection in one case and sudek's atrophy in two cases. The advantages of this surgical interference were restoration of the congruity and height of the nearby joints, in addition to decompression of the lateral structures to prevent the development of impingement syndrome. Also if arthrodesis will be needed later on, it would be done on a near anatomical position

Detection of chlamydia trachomatis DNA by polymerase chain reaction in urine of women with tubal infertility as diagnosed by hysterosalpingography and laparoscopy

This study included 30 women with tubal infertility and 14 women with other causes of infertility documented by laparoscopy. The presence of C. trachomatis DNA in first void urine was determined using PCR assay and the presence of C. trachomatis-specific IgG in serum samples was also investigated using microimmunofluorescence [MIF] technique. C. trachomatis-specific IgG was present in 14 of 30 women with tubal infertility compared with seven of 14 with other causes of infertility. In conclusion, there was a strong association between the presence of C. trachomatis DNA and the development of chlamydial- associated infertility

Oestrus ovis [sheep nasal fly] infesting the eyes and the nose of a camel keeper family

Myiasis is a medical and veterinary health problem in many animal rearing countries. It is not a new problem in Egypt. A family of 5 persons was infested in the eyes [5] and nose [2] with the larvae of Oestrus ovis. It was concluded that the physicians in the MOH rural health units should keep in mind ophthalmomyiasis when dealing with non-specific catarrhal conjunctivitis, particularly those people who are concerned with rearing of farm animals

Effect of various incubation temperatures on in-Vitro intracellular killing of salmonella typhi

The in vitro intracellular killing of Salmonella typhi by human neutrophils under incubation temperatures of 37°C and 40°C was tested at various time intervals. The study employed bactericidal assay. At 37°C the intracellular killing of the organism was high at the first 30 min. then it became stationary up to 120 min. At 40°C the intracellular killing was observed in the first 20 min. which reversed to active multiplication of the organism inside the neutrophil with further incubations up to 120 min

Electrocardiographic study in children with ventricular septal defect

Twenty patients with isolated ventricular septal defect [VSD] were submitted to this study. It aimed at studying the value of clinical, radiological and electrocardiographic aspects of their disease in selecting patients for surgery. They were divided into two groups: Group A, with pulmonary systolic arterial pressure [PSAP] more than 50 mmHg and group B, with PSAP less than 50 mmHg. All patients were subjected to history taking, clinical examination, plain chest X-rays, electrocardiography [ECG] and cardiac catheterization. The results showed that the clinical picture is of no value in detecting the underlying pathophysiology, but radiological findings were found to be useful in estimating the reaction of the pulmonary vasculature to the shunt. Lastly, the ECG proved to have an important role in selecting patient for surgery. The presence of left ventricular hypertrophy [LVH] alone or with right ventricular hypertrophy [RVH] means that the shunt is still left to right and surgical treatment is possible

Auto-immune laboratory tests profile of low frequency syndrome patients

Special form of sensori-neural hearing loss [low frequency syndrome] was studied for autoimmune profile. Routine laboratory tests and antigen nonspecific tests [IgG, IgA, IgM and Clq] were carried out. Detection of autoantibodies [ANA, AMA and ASMA] were also done. There was a striking hyperglobulinemia and detection of autoantibodies. The positive screening tests of an autoimmune profile suggested type II or type III immunologic mechanism of injury

Nitrates and nitrites pollution in cheese and concentrated milk

Twenty five different cheese samples and ten concentrated milk samples were randomly collected from different localities of Zagazig City. Sharkia Governorate. The collected samples were analysed colorimetrically for nitrates and nitrites by using spectrophotometer. The obtained results revealed that the average concentration of nitrates was 86.61, 123.92 and 130.86 mg/kg in skimmed milk, Domiati and full cream soft cheeses samples respectively, while processed and hard cheese samples contained an average concentration of 50.21 and 26.58 mg/kg respectively. The average concentration of nitritesis, skimmed milk, domiati and full cream soft cheese was 1.77, 2.014 and 1.64 mg/kg respectively. Meanwhile, processed and hard cheese exhibited nitrites with an average concentration of 0.81 and 4.27 mg/kg. The examined milk powder and condensed milk samples having an average concentration of 150.62 and 146.32 mg/kg for nitrates, while nitrites was 1.04 and 1.79 mg/kg in milk powder and condensed milk. The most analysed samples contained nitrates above the desirable limit [50 mg/kg] recommended by and Stephany et al. [1978] and Bertelsen [1979]. In contrast nitrites were within the desirable limit [2 mg/kg] in most of the analysed samples with the exception of domiati and hard cheese samples. The results of this study indicate that presence of nitrates and nitrites above the desirable limit in milk and milk products act as heath hazard for man consuming such contaminated products

Hypergammaglobulinemia in chronic liver diseases

Immunoglobulins G, A, and M were measured by the radial immunodiffusion method in the serum of 49 patients with chronic liver diseases and 21 normal controls. The 49 patients were subjected. to histopathological examination. 48 of them were divided according to the results of the this examination into. 5 groups: Goup I] 13 patients with bilharziasis; Group II] 9 patients with bilharziasis plus chronic persistent hepatitis; Group III] 6 patients with bilharziasis plus chornic active hepatitis; Group lV] 7 patients with chronic persistent hepatitis; Group V] 13 patients with chronic active hepatitis. Immunoglobulin levels in the controls were used to calculate the normal immunoglobulin ranges in the Egyptian population. Comparatively abnormal high values of immunoglobulins were found in the patients. In bilharzial patients 75% showed abnormally high levels, and values. > 3600 mg/dl were found in 2 patients. 46.5% showed raised levels of IgA, 2 patients being > 450 mg/dl. 61.5% demonstrated raised levels of IgM, with 3 patients > 475 mg/dl. In bilharziasis plus chronic persistent hepatitis 57% of patients showed a raised level of IgG, with 2 patients > 3600 mg/dl, 28.5% showed values > 450 mg/dl for IgA. 12.5% showed a raised IgM level of 275 mg/dl. 100% of patients with bilharziasis plus chronic active hepatitis showed abnormally high levels of IgG. 20% showed IgA > 450 mg/dl. 80% had a raised level of IgM, 2 patients being] > 475 mg/dl. 28.5% of patients with chronic persistent hepatitis demonstrated a raised level of. IgG, one patients > 3600 mg/dl. 14% showed increased IgA level > 450 mg/dl, 71% showed abnormal levels of IgM with one patient 475 mg/dl. 45% of chronic active hepatitis patients showed a high concentration of IgG, 3 patients > 3600 mg/dl. 45% had abnormally high IgA values, 2 patients] 450 mg/dl; 54.5% showed high levels of IgM. Since 100% and 80% of patients showed high levels of lgG and IgM respectively in bilharziasis plus chronic active hepatitis and IgG and IgM were not highly elevated in patients with bilharziasis plus chronic persistent hepatitis and, chronic persistent hepatitis per se therefore this may serve as a distinguishing factor between both active and chronic hepatitis. Bilharziasis on top of chronic active hepatitis seems to be an additional factor adding to the seriousness of the condition and enhancing hypergammaglobulinaemia which is evident in raised levels of IgG and IgM

Escherichia coli antibodies in patients with chronic liver diseases

49 subjects clinically diagnosed as chronic liver disease patients were subjected to histopathological examination. 48 of them were divided according to the results of tie histopathological examination into 5 groups: Group I-13 patients with bilharziasis; group II-9 patients with bilharziasis plus chronic persistent hepatitis; group III -6 patients with bilharziasis plus chronic active hepatitis; group IV -7 patients with chronic persistent hepatitis ;group V -13 patients with chronic active hepatitis. Using the microtitre haemagglutination technique the serum E. coli O antibodies were significantly high in patients with bilharziasis plus chronic active hepatitis [mean value +/- standard deviation: 219+156]. A statistically significant difference was found when antibody titre +/- standard deviation in group III was compared to that of patients in group I [t =1.6615, P /= 0.05; t = 0.2239, P >/= 0.05] respectively. There was one patient clinically diagnosed as chronic liver disease but whose liver histhpathology proved normal; this patients serum did not demonstrate E. coli O antibodies like the .21 normal controls

Recurrent ventricular tachycardia

Seventeen patients with recurrent ventricular tachycardia were studied. Fourteen had acute cardiac infraction, one with Rh. heart disease, one with hypertension and one had no clinical heart disease. The underlying causes for recurrence were: left ventricular failure in 3 patients, extension of infraction in 3 patients and left ventricular aneurysm in 2 patients. Hypokalemia and thyrotoxicosis were found each in 2 patients while drug toxicity was found in 5 patients: 3 with digitalis, 1 with isoprenalin and 1 with insulin. Diagnosis was established from the surface ECG in 15 patients while in 2 patients it was difficult and in one of them necessitated an intracardiac ECG and in the other patient right atrial pacing was needed to rule out supraventricular tachycardia with aberration. DC shock and xylocaine were successful to stop VT in all patients. Quinidine was the most successful single drug to prevent recurrences in 9 patients. Procaine amide, propranolol were successful each in 2 patients. Combination of quinidine and diphenylhydantion were necessary to prevent the arrhythmia in one patient with LV aneurysm. Correction of underlying cause was mandatory